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QuantiFERON-TB Gold Plus (Single Tube) Testing

QuantiFERON-TB Gold Plus (single tube collection) Specimen Collection, Packaging & Shipping

  1. Required specimen: Whole blood collected in lithium-heparin tube
  2. Minimum volume required: 5 mL
  3. Specimens lacking complete information will not be tested until the missing information has been provided.
  4. Specimens must be received at the lab within 12 hours if shipped at ambient temperature (17-25°C). Samples shipped on ice packs must be shipped to the lab to be received within 48 hours. Tubes must be received cold and should not be frozen.
  1. Lithium-heparin blood collection tubes (must not be expired)
  2. Blood collection supplies
  3. Category B shipping materials (insulated shipper, with ice packs) meeting UN3373 Biological Substances standards

QFT kits can be ordered from the IDOH Containers Department through LimsNet or by emailing

  1. Label the tube with the patient name, DOB, and date and time of collection, including a minimum of two unique identifiers on the tube.
  2. Fill a lithium-heparin blood collection tube with a minimum of 5 mL of blood and gently mix by inverting the tube several times to dissolve the heparin. Tubes should be at room temperature (17-25° C) at the time of collection.
  3. After collection, blood tubes may be held at room temperature (17-25°C) for up to 3 hours prior to refrigeration.
  4. Ensure the collection tube is capped securely. Leaking specimens are unacceptable for testing and will be discarded.
  5. Complete the LimsNet test request for QuantiFERON-TB, ensuring that the patient information on the specimen tube matches the information entered into the submission form.

Ship specimens to:
Indiana Department of Health Laboratory
Attn: TB Lab
550 W. 16th St. - Suite B
Indianapolis, IN  46202

  1. Place the blood collection tubes in the specimen transport bag with absorbent material. Seal the bag using the adhesive at the top.
  2. Store the LimsNet coversheet in the outer pouch of the specimen transport bag or outside of the Styrofoam box to prevent contamination from the specimen.
  3. Place the specimen transport bag and frozen ice packs inside of the Styrofoam box and close the outer shipping box.
  4. The outer shipping box should be labelled using an IDOH 6A label and then placed inside of the UPS Laboratory Pak bag.
  5. Complete the pre-addressed shipping label and affix it to the outer packaging with a return address and appropriate shipping labels. Specimens may be shipped via commercial service, such as UPS or FedEx. Alternatively, specimens may be delivered to IDOH in-person during normal business hours.
  6. Specimens should be shipped to arrive at IDOH Monday through Friday. Specimens should not be shipped to be in transit during the weekend or holiday.
  7. All patient specimens should be labeled, packaged and shipped in accordance with federal shipping regulations, using Biological Substances, Category B.
  1. Results will be reported by LimsNet and will be available in 3-5 business days.
  2. Results will be reported as Positive, Negative or Indeterminate as per the manufacturer's reporting criteria.
  3. Additional information on result interpretation and specimen collection can be found at:

$20, or provide valid insurance information