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Nitrate + Nitrite as N Analysis

All well owners are encouraged to test their water yearly for nitrates. High levels of nitrates may indicate a failing septic system or contamination from animal herds, fertilizers, or landfills. The Nitrate + Nitrite test is a good indicator of overall water quality. Using Federal regulations under the Safe Drinking Water Act (40 CFR 141.23 (a) (4) (i)) as a guideline, the maximum contaminant level (MCL) for Nitrate as Nitrogen is 10.0 mg/L (ppm) and for Nitrite as Nitrogen is 1.0 mg/L (ppm). The health significance of nitrate above these levels applies to pregnant women, and infants below the age of six months. The major health effect associated with these levels is methemoglobinemia, also known as “blue-baby syndrome”.

Samples will be accepted from the public for a fee of $10.00 per sample plus $7.00 shipping and handling per order.

Samples from State government agencies, State Institutions and County Health Departments will be accepted at no charge.

Contact: Bharat Patel
Phone: 317-921-5586

$25 per sample plus shipping for Public Water Utilities
No Charge for Investigations by Local Health Departments, IDEM or other state agencies and institutions


The kit contains:

  • Instruction form
  • Analysis request form
  • One 125mL plastic bottle with a filter paper and a small amount of Sulfuric Acid.

Order kits using the link below:
Access Indiana: Home

    Do not rinse the sample container or discard the acid preservation disk! The filter disk is saturated with 50% sulfuric acid (see precautions) and is used to preserve the sample for the analysis of total nitrate/nitrite. Flush the sample tap for five (5) minutes prior to collecting the sample. Reduce the flow of the water and fill the sample container to the shoulder of the bottle. Replace and tighten the cap to prevent sample leaking. Do not take the filter paper out. Complete the analysis request form.

Order kits using the link below:
Access Indiana: Home
Include Chain of Custody
Shipping address is:

IDOH Laboratory
Suite B
550 W 16th St
Indianapolis, IN 46202

The report will be emailed/faxed or mailed within 21 days after receiving the sample.